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Rapido PCR termici utilizzando microscala convezione termica
JoVE Journal
Biologie
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JoVE Journal Biologie
Rapid PCR Thermocycling using Microscale Thermal Convection
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Rapido PCR termici utilizzando microscala convezione termica

22,396 Views

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09:02 min

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March 05, 2011

DOI:

10.3791/2366-v

DOI:
10.3791/2366-v

•

09:02 min
March 05, 2011

•

7 Views
, ,
1Department of Mechanical Engineering,Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering,Texas A&M University, 3Department of Chemical Engineering,Texas A&M University

Summary

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Descriviamo un nuovo metodo per eseguire la replicazione del DNA tramite la reazione a catena della polimerasi (PCR). Convezione termica viene sfruttata per reagenti navetta continuo tra denaturazione, ricottura, e le condizioni di estensione, mantenendo opposte superfici del reattore a temperatura costante. Questo design intrinsecamente semplice promette di rendere rapido PCR più accessibile.

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Cite this Article

Muddu, R., Hassan, Y. A., Ugaz, V. M. Rapid PCR Thermocycling using Microscale Thermal Convection. J. Vis. Exp. (49), e2366, doi:10.3791/2366 (2011).

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