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快速PCR热循环利用微型热对流

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Rapid PCR Thermocycling using Microscale Thermal Convection

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快速PCR热循环利用微型热对流

22,396 Views

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09:02 min

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March 05, 2011

DOI:

DOI:

•

09:02 min

March 05, 2011

•

7 Views

Radha Muddu¹, Yassin A. Hassan², Victor M. Ugaz³

¹Department of Mechanical Engineering,Texas A&M University, ²Department of Mechanical Engineering and Department of Nuclear Engineering,Texas A&M University, ³Department of Chemical Engineering,Texas A&M University

Summary

我们描述了一种新的方法，通过聚合酶链反应（PCR）进行DNA复制。热对流是利用变性，退火，以及延长条件之间不断穿梭试剂保持反对在反应器的表面温度恒定。这在本质上简单设计的承诺，使快速PCR更方便。

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