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Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
JoVE Journal
Biologie
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JoVE Journal Biologie
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

DOI:
10.3791/2362-v

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07:53 min

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December 10, 2010

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, , , , , ,
1Orthopaedics Research,University of Virginia, 2Biological Sciences,University of Delaware, 3Orthopaedic Surgery,University of Virginia

Kapitel

  • 00:05Titel
  • 01:44Biopanning
  • 03:39Peptide Staining of Mesenchymal Cultured Cells
  • 04:42Preparation of Gel Foam Composite and In Vivo Implantation
  • 05:25Histology
  • 05:40Effects of Phage Display Peptides on Osteogenesis In Vitro and In Vivo
  • 06:40Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these peptides on mesenchymal cell differentiation and to determine their effect on bone regeneration.

Tags

PeptidesPhage Display LibraryGene ExpressionMesenchymal CellsOsteogenesisUnicortical Bone DefectsBone FormationCollagen MatrixAlkaline Phosphatase ActivityNodule FormationRunx2OsterixBone SialoproteinOsteocalcinCollagen SpongeRepair Of Bone DefectsIn Vitro DifferentiationIn Vivo Delivery

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