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Yapışkan bant tabanlı Örnekleme ve Floresans kombinasyonu In situ Hibridizasyon Salmonella Taze üretin
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Yapışkan bant tabanlı Örnekleme ve Floresans kombinasyonu In situ Hibridizasyon Salmonella Taze üretin

12,817 Views

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09:10 min

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October 18, 2010

DOI:

10.3791/2308-v

DOI:
10.3791/2308-v

•

09:10 min
October 18, 2010

•

6 Views
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1Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Summary

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Bu protokol, ardından hızlı bir şekilde tüm hücre algılama, domates ve diğer taze ürün yüzeyler örnekleme için basit bir yapışkan bant tabanlı yaklaşım, açıklar<em> Salmonella</em> Floresan kullanarak<em> In situ</em> Hibridizasyon (FISH).

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Adhesive-tape-based SamplingFluorescence In Situ HybridizationRapid DetectionSalmonellaFresh ProduceCulture-independent DetectionCell-charged TapesSolid-phase EnrichmentLiquid Surface MinicultureDNA Probe CocktailHybridizationFluorescence Microscopy

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Cite this Article

Bisha, B., Brehm-Stecher, B. F. Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce. J. Vis. Exp. (44), e2308, doi:10.3791/2308 (2010).

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