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Retrovirale Transduktion von T-Zell-Rezeptoren in Maus-T-Zellen
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Kapitel
- 00:00Titel
- 01:37Retroviral Transfection and T-cell Isolation
- 03:55Prepapration of a Single-cell Suspension from Mouse Spleen
- 04:51Isolation and Activation of Mouse T-cells
- 06:27Infection of Activated T-cells
- 08:21Expansion of Cells and Evaluation of Transduction Efficiency
- 08:55Retroviral Expression of TCR on Mouse T-cells
- 09:32Conclusion
Tags
Retroviral TransductionT-cell ReceptorsMouse T-cellsImmune SystemPeptide AntigensAntigen Presenting CellsFunctional OutcomesImmunology Related DiseasesTCR Transgenic Mouse ModelsBone Marrow ChimeraRetroviral Transduction MethodPeptide-MHC SpecificityDownstream ApplicationsHuman ImmunotherapyAdoptive TransferCancer Treatment
