Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations
Transkript
Take a drop of fresh mouse urine and immerse a glucose detection strip containing glucose oxidase, peroxidase, and chromogen.
Glucose oxidase oxidizes urine glucose, forming gluconolactone and hydrogen peroxide.
Further, peroxidase utilizes hydrogen peroxide to catalyze the chromogen's oxidation, forming a colored product, with intensity proportional to urine glucose concentration.
Compare the color with the strip's reference chart for qualitative assessment of urine glucose.
For quantitative urine glucose measurement, take a multi-well plate with glucose concentration standards and the mouse urine sample collected over a defined period.
Add an assay mixture containing glucose oxidase, peroxidase, and indicator coupling reagents.
Glucose oxidase facilitates the oxidation of glucose, forming gluconolactone and hydrogen peroxide. Peroxidase catalyzes the reaction between coupling reagents and hydrogen peroxide, forming a pink-colored product.
Using a microplate reader, measure the absorbance of the colored product.
With the generated glucose standard curve, determine the glucose concentration in the urine.
