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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
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JoVE Journal Biologie
Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

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10:55 min

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December 07, 2010

DOI:

10.3791/2166-v

DOI:
10.3791/2166-v

•

10:55 min
December 07, 2010

•

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1Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics,University of Pennsylvania-School of Medicine

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Immunofluorescent DetectionThymidine AnaloguesCldUIdUCell Division MeasurementExperimental BiologyMicroscopyVital DyesCFSEKi67PCNABromo-deoxyuridine (BrdU)Chloro-deoxyuridine (CldU)Iodo-deoxyuridine (IdU)Ethinyl-deoxyuridine (EdU)Radio-detectionImmuno-detectionChemical DetectionAdult MiceImmunostaining ProtocolsCell Turnover

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Tuttle, A. H., Rankin, M. M., Teta, M., Sartori, D. J., Stein, G. M., Kim, G. J., Virgilio, C., Granger, A., Zhou, D., Long, S. H., Schiffman, A. B., Kushner, J. A. Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue. J. Vis. Exp. (46), e2166, doi:10.3791/2166 (2010).

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