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Bacterial Gene Expression Analysis Using Microarrays

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Biologie

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JoVE Journal Biologie

Bacterial Gene Expression Analysis Using Microarrays

Bacterial Gene Expression Analysis Using Microarrays

DOI:

•

29:41 min

•

May 28, 2007

•

Sinem Beyhan, Fitnat Yildiz

¹Department of Environmental Toxicology,University of California Santa Cruz – UCSC

Kapitel

00:04Introduction
00:24cDNA synthesis
02:02cDNA purification
08:56Fluorescent labeling of cDNA
10:09Determining concentration of fluorescent probe with Nanodrop
11:42Pre-hybridization
14:46Preparation of hybridization sample
19:28Hybridization
24:51Washing
28:02Scanning

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Bacterial Gene Expression Analysis Microarrays

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