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만성 두 광자를위한 박막 해골 창 기법 생체내에서 이미징
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
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만성 두 광자를위한 박막 해골 창 기법 생체내에서 이미징

23,111 Views

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12:07 min

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September 19, 2010

DOI:

10.3791/2059-v

DOI:
10.3791/2059-v

•

12:07 min
September 19, 2010

•

11 Views
, , , ,
1Center for Neural Development and Disease, Department of Neurology, Child Neurology Division,University of Rochester, 2Department of Neurobiology and Anatomy,University of Rochester

Summary

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우리는 반복 murine microglia를 시각화하고 monocytes 순환하는 방법을 설명<em> 생체내에</em> 시간, 일 또는 transcranial 두 광자 현미경을 사용하여 주 동안. 우리는 HIV - 1 단백질 규제 태트의 인접한 stereotactic 분사에 의해 활성화될 수있는 정지 microglia의 간헐적인 관찰을 허용 thinned - 해골 창을 준비하는 방법을 보여줍니다.

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Marker, D. F., Tremblay, M., Lu, S., Majewska, A. K., Gelbard, H. A. A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation. J. Vis. Exp. (43), e2059, doi:10.3791/2059 (2010).

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