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Quantifying DNA Double Strand Break Repair with Nanoluciferase Reporter Assays
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Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways
JoVE Journal
Krebsforschung
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JoVE Journal Krebsforschung
Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways

Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways

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03:21 min

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June 14, 2024

DOI:

10.3791/202011-v

DOI:
10.3791/202011-v

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03:21 min
June 14, 2024

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1Artios Pharma Ltd, Cambridge, 2The Francis Crick Institute, London

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KrebsforschungNon-homologous End JoiningMicrohomology-mediated End JoiningSingle Strand AnnealingNanoluciferase Reporter AssayHigh-throughput ScreeningDNA Repair ResearchDrug Discovery

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Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways. J. Vis. Exp. (Pending Publication), e202011, doi:10.3791/202011 (2024).

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