Journal
/
Neurowissenschaften
/
Flow Cytometry-Based Assays for Microglial Engulfment of Synaptic Materials
/
Flow Cytometric Quantification of In Vitro Microglial Engulfment of Crude Synaptosomes for the Analysis of Synaptic Refinement
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal Neurowissenschaften
Flow Cytometric Quantification of In Vitro Microglial Engulfment of Crude Synaptosomes for the Analysis of Synaptic Refinement

Flow Cytometric Quantification of In Vitro Microglial Engulfment of Crude Synaptosomes for the Analysis of Synaptic Refinement

113 Views

•

03:15 min

•

May 31, 2024

DOI:

10.3791/201772-v

DOI:
10.3791/201772-v

•

03:15 min
May 31, 2024

•

1 Views
,
1Psychoneuroimmunology,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, 2Experimental Ophthalmology,Charité Universitätsmedizin Berlin

Explore More Videos

Flow CytometryMicroglial EngulfmentSynaptosomesSynaptic RefinementCell SuspensionPercoll Gradient CentrifugationMACS BufferMagnetic SeparatorPHrodo Red-labeled SynaptosomesTrypsin-EDTAFACS BufferCD11bCD45Cell StainingFluorescence Analysis

Read Article

Cite this Article

Flow Cytometric Quantification of In Vitro Microglial Engulfment of Crude Synaptosomes for the Analysis of Synaptic Refinement. J. Vis. Exp. (Pending Publication), e201772, doi:10.3791/201772 (2024).

Download .ris file

Copy

Share Video

.

Copy