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Biochemie
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Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
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Affinity Column Preparation and Packing for Fast Protein Liquid Chromatography (FPLC)
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Biochemie
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JoVE Journal Biochemie
Affinity Column Preparation and Packing for Fast Protein Liquid Chromatography (FPLC)

Affinity Column Preparation and Packing for Fast Protein Liquid Chromatography (FPLC)

Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein

DOI:
10.3791/201753-v

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01:42 min

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April 26, 2024

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, ,
1Department of Biology,Indiana University Indianapolis

Tags

6X-His-tagged ProteinAffinity PurificationFast Protein Liquid Chromatography (FPLC)Flap Endonuclease 1 (FEN1)Protein ExpressionProtein PurificationProtein StabilityProtein FunctionalitySample PreparationColumn PackingFlow RateElution ParametersChromatogram AnalysisRecombinant Protein Storage

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