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Journal
/
Biochemie
/
Development of a Click-Based Assay for Screening HAT1 Enzyme Activity
/
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
JoVE Journal
Biochemie
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JoVE Journal
Biochemie
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
Development of a Click-Based Assay for Screening HAT1 Enzyme Activity
DOI:
10.3791/201342-v
•
04:18 min
•
January 26, 2024
•
Shreenidhi Rajkumar
,
Danielle Dixon
,
Andrew M. Lipchik
,
Joshua J. Gruber
1
Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences
,
University of Texas Southwestern Medical Center
,
2
Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences
,
Wayne State University
Tags
Click Assay
Histone Acetyltransferase 1 Acetylation
Biotinylated Histone H4 Peptide
Acetylation Reactions
4-Penten Oil Co-enzyme A
BSA Pre-blocked Black NeutrAvidin-coated 96-well Plates
THPTA Copper Mixture
Sodium Ascorbate
Biotin Azide
Streptavidin Horseradish Peroxidase
Amplex Red Detection Reagents
Hydrogen Peroxide
Sodium Phosphate
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Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
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