Journal
/
Entwicklungsbiologie
/
Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
/
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:
10.3791/200860-v

•

02:37 min

•

November 10, 2023

•

, , , , , ,
1Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, 2Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, 3Department of Hematology,Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Tags

GutZebrafish LarvaeFACSCell IsolationDissociationPapainAgarose PlateHEPESE3 MediumDissectionIntestineCell StrainerCentrifugationDAPICell Labeling

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq

Read Article