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Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

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Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival

JoVE Journal
Genetik

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JoVE Journal Genetik

Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival

Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival

Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

DOI:

10.3791/200841-v

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01:40 min

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December 01, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka, Satoru Kobayashi, Toshiyuki Takano-Shimizu-Kouno

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Tags

Cryopreserved Primordial Germ Cells Drosophila Strain Revival Embryo Transplantation PGC Thawing Capillary Holder Posterior Pole Injection Vitelline Membrane Somatic Cell Layer Strain Revival

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