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Journal
/
Entwicklungsbiologie
/
Investigating Development with Optogenetic Signaling Manipulation in Zebrafish
/
Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos
Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos
Investigating Development with Optogenetic Signaling Manipulation in Zebrafish
DOI:
10.3791/200757-v
•
01:56 min
•
October 27, 2023
•
Allison J. Saul*
1
,
Catherine E. Rogers*
1
,
Marcial Garmendia-Cedillos
,
Thomas Pohida
,
Katherine W. Rogers
1
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development
,
NIH
,
2
Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering
,
NIH
Tags
Zebrafish Embryos
Opto-BMP-Nodal Constructs
Light Box
LED Microplate Illuminator
Injection Dishes
Agarose-coated Plates
Glass Pipettes
Immunofluorescence
Phenotyping Assay
Developmental Stages
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Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions
Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos
Light Exposure Experiment to Activate BMP or Nodal Signaling in Zebrafish
Evaluation of bOpto-BMP/Nodal Expressing Embryos After the Light Exposure Experiment
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