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Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

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Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster

JoVE Journal
Biochemie

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JoVE Journal Biochemie

Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster

Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster

Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

DOI:

10.3791/200717-v

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01:38 min

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November 10, 2023

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Paula Michelotti*^1,2, Tâmie Duarte*^1,2, Cristiane L. Dalla Corte²

¹Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences,Federal University of Santa Maria, ²Graduate Program in Biological Sciences: Toxicological Biochemistry,Federal University of Santa Maria

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Oxygen Sensor Polarographic Drosophila Melanogaster High-resolution Respirometry MIRO Five Buffer Oroboros Chamber Oroboros DatLab Software Chamber Calibration Temperature Barometric Pressure

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