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Journal
/
Genetik
/
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
/
Sonication and Biotin Pull-Down of Scarce Cells DNA
JoVE Journal
Genetik
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JoVE Journal
Genetik
Sonication and Biotin Pull-Down of Scarce Cells DNA
Sonication and Biotin Pull-Down of Scarce Cells DNA
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
DOI:
10.3791/200138-v
•
01:47 min
•
April 21, 2023
•
Llorenç Rovirosa*
1
,
Laureano Tomás-Daza*
1,2
,
Blanca Urmeneta
,
Alfonso Valencia
3
,
Biola M. Javierre
4
1
Josep Carreras Leukaemia Research Institute
,
2
Barcelona Supercomputing Center
,
3
Catalan Institution for Research and Advanced Studies (ICREA)
,
4
Institute for Health Science Research Germans Trias i Pujol
Tags
Sonication
Biotin Pull-down
Scarce Cells DNA
Ligated And Decross-linked Digested Chromatin
Water Bath Sonicator
End Repair
Streptavidin Beads
Tween Buffer
No-Tween Buffer
DNA Fragments
Embed
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Verwandte Videos
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Lysis and Digestion of Scarce Cells
Ligation and Decrosslinking of Scarce Cell DNA
Sonication and Biotin Pull-Down of Scarce Cells DNA
DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library
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