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技术成像钙^{2 +}信号在人类精子

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Techniques for Imaging Ca²⁺ Signaling in Human Sperm

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技术成像钙^{2 +}信号在人类精子

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07:38 min

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June 16, 2010

DOI:

DOI:

•

07:38 min

June 16, 2010

•

12 Views

Katherine Nash¹, Linda Lefievre², Ruben Peralta-Arias¹, Jennifer Morris¹, Aduen Morales-Garcia¹, Tom Connolly², Sarah Costello¹, Jackson C. Kirkman-Brown³, Stephen J. Publicover¹

¹School of Biosciences,University of Birmingham, ²School of Medicine,University of Birmingham, ³Centre for Human Reproductive Science,Birmingham Women’s Hospital

Summary

刺激诱发的[Ca<sup> 2 +</sup>]：我个人的人类精子的信号进行评估。运动型细胞加载与Ca<sup> 2 +</sup>敏感的荧光染料（AM -酯法），并固定在perfusable室。时间推移荧光显微镜和细胞成像，通过灌注介质刺激。单细胞的反应（或地区）使用Excel进行离线分析。

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