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Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
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Biologie
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JoVE Journal Biologie
Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

DOI:
10.3791/1700-v

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09:21 min

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June 17, 2010

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, ,
1Thermo Scientific Solaris qPCR Products

Kapitel

  • 00:00Titel
  • 01:00Introduction
  • 01:25The Solaris qPCR Gene Expression Assay Design Algorithm
  • 03:05Planning the qPCR Experiment
  • 04:41qPCR Setup
  • 07:51Representative qPCR Results Using Solaris
  • 08:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The Solaris qPCR Gene Expression Assays are novel pre-designed qPCR primer/probe combinations designed to simplify the qPCR process without sacrificing the specificity and robustness of the assay.

Tags

Quantitative Real-Time PCRThermo Scientific Solaris QPCR AssayPrimer/probe SetQPCR ProcessSensitivityAccuracyPre-designed Primer/probe SetsHuman GenomeMouse GenomeDesign AlgorithmBioinformatics ExpertsConvenient FeaturesStreamline ProcessMaster MixThermal Cycling ConditionsProbe And Primer Sequence InformationGENEius Product Search FeatureOrdering Web SiteStandard Curve Method

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