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1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard
Kapitel
- 00:00Titel
- 00:05Introduction
- 00:44Procedure Overview
- 01:45Day 1: Coating of Glass Coverslips and Heat Sterilization Tools
- 02:41Day 2: Isolation and Culture of the Murine Organ of Corti
- 08:15Applications for Isolated Organ of Corti
- 11:08Day 4: Visualization of Electroporated Cells
- 11:37Conclusion
Dieses Verfahren beschreibt eine Methode zur Isolierung und Kultivierung der murinen Corti-Organ mit oder ohne die Spirale Limbus und Spiralganglions Neuronen. Wir zeigen auch ein Verfahren zur Expression eines exogenen Reporter-Gen in das Corti-Organ Explantat durch Elektroporation.
Tags
Primary CulturePlasmid ElectroporationMurine Organ Of CortiSensory EpitheliumAuditionCochleaInner EarHair CellsMechanosensory CellsInner Hair CellsOuter Hair CellsBasilar MembraneSensorineural Hearing LossAuditory ResearchOrgan ExplantGene ExpressionStereocilia MotilityCell And Molecular BiologyHair Cell RegenerationIsolation And Culture MethodNeonatal MiceTemporal Bone Isolation
