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Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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Biologie
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JoVE Journal Biologie
Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

17,117 Views

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05:52 min

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November 19, 2009

DOI:

10.3791/1659-v

DOI:
10.3791/1659-v

•

05:52 min
November 19, 2009

•

7 Views
, ,
1Department of Biomedical Engineering, Faculty of Electrical Engineering,University of Ljubljana

Summary

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External electric field induces a voltage on the membrane of a cell, termed the induced membrane voltage (ΔΦ). By using the potentiometric dye di-8-ANEPPS, it is possible to measure the ΔΦ noninvasively. This video shows the protocol for measuring ΔΦ using di-8-ANEPPS.

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Cite this Article

Pucihar, G., Kotnik, T., Miklavčič, D. Measuring the Induced Membrane Voltage with Di-8-ANEPPS. J. Vis. Exp. (33), e1659, doi:10.3791/1659 (2009).

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