We present a graphene array-based brain mapping procedure to reduce the invasiveness and improve spatiotemporal resolution. Graphene array-based surface electrodes exhibit long-term biocompatibility, mechanical flexibility, and suitability for brain mapping in a convoluted brain. This protocol allows for constructing multiple forms of sensory maps simultaneously and sequentially.
Cortical maps represent the spatial organization of location-dependent neural responses to sensorimotor stimuli in the cerebral cortex, enabling the prediction of physiologically relevant behaviors. Various methods, such as penetrating electrodes, electroencephalography, positron emission tomography, magnetoencephalography, and functional magnetic resonance imaging, have been used to obtain cortical maps. However, these methods are limited by poor spatiotemporal resolution, low signal-to-noise ratio (SNR), high costs, and non-biocompatibility or cause physical damage to the brain. This study proposes a graphene array-based somatosensory mapping method as a feature of electrocorticography that offers superior biocompatibility, high spatiotemporal resolution, desirable SNR, and minimized tissue damage, overcoming the drawbacks of previous methods. This study demonstrated the feasibility of a graphene electrode array for somatosensory mapping in rats. The presented protocol can be applied not only to the somatosensory cortex but also to other cortices such as the auditory, visual, and motor cortex, providing advanced technology for clinical implementation.
A cortical map is a set of local patches representing response properties to sensorimotor stimuli in the cerebral cortex. They are a spatial formation of neural networks and enable prediction for perception and cognition. Therefore, cortical maps are useful in evaluating neural responses to external stimuli and processing sensorimotor information1,2,3,4. Invasive and noninvasive methods are available for cortical mapping. One of the most common invasive methods involves the use of intracortical (or penetrating) electrodes for mapping5,6,7,8.
Assessing the on-demand high-resolution cortical maps using penetrating electrodes has faced several obstacles. The method is too laborious to obtain a decent map and too invasive to implement for clinical use, prohibiting further development. More recent technologies such as electroencephalography (EEG), positron emission tomography (PET), magnetoencephalography (MEG), and functional magnetic resonance imaging (fMRI) have gained popularity because these are less invasive and reproducible. However, given their prohibitive costs and poor resolution, they are used in a limited number of cases9,10,11. Recently, flexible surface electrodes with superior signal reliability have attracted considerable attention. Graphene-based surface electrodes demonstrate long-term biocompatibility and mechanical flexibility, providing stable recordings in a convoluted brain12,13,14,15,16. Our group has recently developed a graphene-based multichannel array for high-resolution recording and site-specific neurostimulation on the cortical surface. This technology allows us to keep track of the cortical representations of sensory information for an extended period.
This article describes the steps involved in acquiring a brain map of the somatosensory cortex using a 30-channel graphene multielectrode array. To measure brain activity, a graphene electrode array is placed on the subdural area of the cortex, while the forepaw, forelimb, hind paw, hindlimb, trunk, and whiskers are stimulated with a wooden stick. The somatosensory-evoked-potentials (SEPs) are recorded for somatosensory areas. This protocol can also be applied to other brain areas, such as the auditory, visual, and motor cortex.
All animal-handling procedures were approved by the Institutional Animal Care and Use Committee of the Incheon National University (INU-ANIM-2017-08).
1. Animal preparation for surgery
NOTE: Use Sprague Dawley Rat (8-10 weeks old) without the sex bias for this experiment.
2. Surgery for cortical surface exposure
3. Preparation of graphene electrode array connected to the recording system
4. Physical stimulation and recording SEPs for mapping
5. Animal euthanasia
6. SEP measurement for cortical mapping
This protocol describes how a graphene multichannel array is mounted on the surface of the brain. The somatosensory map was constructed by acquiring neural responses to physical stimuli and calculating the amplitude of the response. Figure 1 shows the schematic of this experiment.
Figure 2A presents the structural characteristics of a graphene electrode array. There are thru-holes of the substrate between the electrodes. These holes help the electrode firmly contact the cortical surface (Figure 2B). The strong adhesion of the electrode to the cortex helps record neural signals with less noise.
Figure 2C (left) shows the location-dependent neural responses acquired by stimulating the whiskers, trunk, paws, and limbs coded in different colors. A rat homunculus, the miniature body of the rat, is drawn with the actual ratio of each color size in the somatosensory cortex map (Figure 2C, right).
Figure 2D presents stimuli-specific responses with colors associated with each body part. The responses are recorded through a graphene electrode array placed on the surface of the cortex. Using the data recorded from the graphene array, the amplitude of SEPs is calculated to obtain the amplitude-dependent somatosensory map.
Sensory stimulus-induced local field potentials enable the construction of the somatosensory map. The response size to each body stimulus poses rodent homunculus. Each color represents a different body part (Figure 3).
The acquired cortex map using this protocol reveals the specific regions within the somatosensory cortex that respond to the whiskers, forepaws, forelimbs, hind paws, hindlimbs, and trunks. It provides insights into the extent of the involvement of the cortical area in processing physical stimulus information for each body part.
Figure 1: Schematic of the experiment setup. The graphene-based electrode array is attached to the somatosensory cortex, and the whiskers or other body parts are stimulated by gentle touch. The thick red line represents the cable, and the thin red and blue lines represent the ground and reference wires. The black dot indicates the bregma. The data acquisition system is connected to the computer via USB. Please click here to view a larger version of this figure.
Figure 2: Graphene-based microelectrode array for brain mapping on the cortical surface. (A) Schematic of the graphene-based electrode array. (B) Optical image of the graphene electrode array on the cortical surface. (C) Rat's auditory and somatosensory cortices. Two maps of auditory and somatosensory areas responding to auditory stimuli with various frequency tones and physical stimuli applied to each body part. (D) The 30-channel (excluding the reference and ground electrodes) recording of the graphene-electrode array on the cortical surface. Box colors correlate with the geographical locations of the cortical surface. The figures are adapted and modified from Lee et al. (2021).4 Please click here to view a larger version of this figure.
Figure 3: Somatosensory map. (A) Location of neural recordings across cortical layers (left). A cortical surface map determined using a graphene electrode array. A color-coded somatosensory map constructed using the response amplitudes and overlapped with the homunculus (right). (B) Recorded cortical SEPs and maps following the stimulation of each body part. This figure is adapted and modified from Lee et al. (2021).4 Please click here to view a larger version of this figure.
The presented protocol provides an in-depth, step-by-step process that explains how to access and map the somatosensory responses of rats using a graphene electrode array. The protocol-acquired data are SEPs that provide somatosensory information that is synaptically linked to each body part.
Several aspects of this protocol should be considered. When extracting cerebrospinal fluid to prevent brain edema and mitigate inflammation, it is crucial for the experimenter not to damage the brainstem located in front of the cisterna magna.
Facial whiskers provide tactile sensory information about the surroundings, such as a dark and narrow environment. Accordingly, rodent whiskers are well-developed enough to sense an object through the deflection directions, stimulus intensity, and location of the stimulated whiskers. The somatosensory cortex responds to the bending direction, intensity, and location of each whisker differently18,19. Therefore, all whiskers are stimulated with constant intensity and direction in this protocol.
This protocol cannot record signals evoked in deep brain structures as our graphene electrode array is mounted on the cortical surface. Thus, the experimenter cannot identify how the columnar network is hierarchically organized concerning neural responses.
This protocol is superior to previous recording methods because the graphene electrode array is less invasive, adaptable, and biocompatible12,13,14,15,16. Furthermore, the graphene electrode array has >30 channels for recording signals, thus enabling faster cortical mapping than a single or tetrode electrode. This protocol can be further applied to other cortical areas whenever required15,20. The experimenter can place the electrode array on the auditory or visual cortex to extract auditory and visual information as the auditory or visual maps. Finally, this method can be implemented for chronic implantation and diagnosis of neural diseases, such as stroke, epilepsy, tinnitus, and Parkinson's disease.
The authors have nothing to disclose.
This work was supported by Incheon National University (International Cooperative) for Sunggu Yang.
1mL syringe | KOREAVACCINE CORPORATION | injecting the drug for anesthesia | |
3mL syringe | KOREAVACCINE CORPORATION | injecting the drug for anesthesia | |
Bone rongeur | Fine Science Tools | 16220-14 | remove the skull |
connector | Gbrain | Connect graphene electrode to headstage | |
drill | FALCON tool | grind the skull | |
drill bits | Osstem implant | grind the skull | |
Graefe iris forceps slightly curved serrated | vubu | vudu-02-73010 | remove the tissue from the skull or hold wiper |
graphene multielectrode array | Gbrain | records signals from neuron | |
isoflurane | Hana Pharm Corporation | sacrifce the subject | |
ketamine | yuhan corporation | used for anesthesia | |
lidocaine(2%) | Daihan pharmaceutical | local anesthetic | |
Matlab R2021b | Mathworks | Data analysis Software | |
mosquito hemostats | Fine Science Tools | 91309-12 | fasten the scalp |
ointment | Alcon | prevent eye from drying out | |
povidone | Green Pharmaceutical corporation | disinfect the incision area | |
RHS 32ch Stim/Record headstage | intan technologies | M4032 | connect connector to interface cable and contain intan RHS stim/amplifier chip |
RHS 6-ft (1.8m) Stim SPI interface cable | intan technologies | M3206 | connect graphene electrode to headstage |
RHS Stim/Recording controller software | intan technologies | Data Acquisition Software | |
RHS stimulation/ Recording controller | intan technologies | M4200 | |
saline | JW Pharmaceutical | ||
scalpel | Hammacher | HSB 805-03 | |
stereotaxic instrument | stoelting | fasten the subject | |
sterile Hypodermic Needle | KOREAVACCINE CORPORATION | remove the dura mater | |
Steven Iris Tissue Forceps | KASCO | 50-2026 | remove the dura mater |
surgical blade no.11 | FEATHER | inscise the scalp | |
surgical sicssors | Fine Science Tools | 14090-09 | inscise the scalp and remove the dura mater |
wooden stick | whisker stimulation | ||
xylazine | Bayer Korea | used for anesthesia |