Staining of paraffin sections
Antigen recovery:
Material Name | Typ | Company | Catalogue Number | Comment |
---|---|---|---|---|
Xylene | Fisher | X3S-4 | Change xylene every month depending on use. Xylene is TOXIC. | |
100% ethanol | Fisher | A406-20 | Use to make EtOH gradient. | |
DPBS | Dulbecco’s modified Phosphate Buffered Saline WITH Ca2+ and Mg2+ | |||
Na-Citrate buffer | 10 mM, pH 6.5 | |||
H2O2 | Sigma | H-1009 | 30% stock => Dilute to 1% in DPBS | |
Blocking solution | DPBS + 5% bovine serum albumin (BSA) + 0.1% Na Azide + 5% serum. | |||
BSA | ||||
Sodium Azide | NaAzide | |||
avidin/biotin blocking kit | Vector laboratories | SP-2001 | ||
Ab buffer | DPBS + 2% BSA + 0.1% Na Azide + 2% serum (same as used for the blocking step) | |||
ABC kit Vectastain PK-6100 | Vector Laboratories | |||
DAB peroxidase substrate | Vector Laboratories | SK-4100 | ||
ddH2O | ||||
Hematoxylin | Fisher | CS401-D | counterstain | |
Permount | Fisher | SP15-100 | ||
Slides and cover-slips | glass |