Retinal Cup Extraction from Mouse: A Surgical Procedure to Obtain Intact Retinal Cup from Mouse Eye

Published: April 30, 2023

Abstract

Source: Jiang, K. et al. Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples. J. Vis. Exp (2021)

This video describes a protocol to isolate the retinal cup without the outer covering layers from the eyeball of a mouse. The extracted retinal tissue can help study various metabolic pathways occurring in the retinal cells.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Retinal cup dissection Euthanize a mouse by CO2 asphyxiation following institutional guidelines on euthanasia. NOTE: Do not leave the animal in a CO2 chamber longer than the time needed for euthanasia. Enucleate eyes and place into ice-old 1x PBS buffer …

Offenlegungen

The authors have nothing to disclose.

Materials

1X PBS Thermo Fisher 14190-144
30-gauge needle BD Precision Glide 305106
Biopsy puncher, 1 mm Integra Miltex 33-31AA
CO2 asphyxiation chamber
Dissection forceps-Dumont #5 Fine Science Tools 11251-10 Stright tip
Dissection forceps-Dumont #7 Fine Science Tools 11274-20 Curved tip
Dissection microscope
Graefe forceps Fine Science Tools 11051-10 Curved, Serrated tip
Microscissors Fine Science Tools 15004-08 Curved tip

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Retinal Cup Extraction from Mouse: A Surgical Procedure to Obtain Intact Retinal Cup from Mouse Eye. J. Vis. Exp. (Pending Publication), e20800, doi: (2023).

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