This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.
Protocol
1. Decellularization NOTE: This procedure was adapted from previously published methods focused on adipose decellularization, which included the sodium deoxycholate ionic detergent rather than sodium dodecyl sulfate to remove DNA efficiently. On day 1, remove frozen Mammary Fat pads or MFPs from -80 °C and thaw at room temperature. Once thawed, dry MFPs briefly on a delicate task wipe. Weigh the MFPs using an analytical …