JOVE JOURNAL

Immunologie und Infektion

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Journal (Immunology and Infection)

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Emily Morgan¹, Jonah Faye Longares¹, Marie-Anne Félix², Robert J. Luallen¹

¹Department of Biology,San Diego State University, ²Institut de Biologie de l’École Normale Supérieure

Journal (Immunology and Infection)

Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery

Guy El Helou¹, Joseph F. Goodman², Maria Blevins³, David L. Caudell⁴, Todd A. Ponzio³, John W. Sanders^3,5

¹Department of Medicine, Division of Infectious Diseases and Global Medicine,University of Florida, ²Department of Otolaryngology,George Washington University School of Medicine, ³Department of Medicine, Section on Infectious Diseases,Wake Forest Baptist Medical Center, ⁴Department of Pathology,Wake Forest School of Medicine, ⁵Department of Medicine, Section of Infectious Diseases,Hefner Veterans Affairs Medical Center

Journal (Immunology and Infection)

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Reza Rezaei^1,2, Abera Surendran^1,2, Ragunath Singaravelu^1,2, Taylor R. Jamieson^1,2, Parisa Taklifi³, Joanna Poutou^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ³Department of Biotechnology,University of Tehran

Journal (Immunology and Infection)

Assessing Biofilm Dispersal in Murine Wounds

Whitni K. Redman*^1,2, Garrett S. Welch*^1,3, Kendra P. Rumbaugh^1,2,3

¹Department of Surgery,Texas Tech University Health Sciences Center, ²Immunology and Molecular Microbiology,Texas Tech University Health Sciences Center, ³TTUHSC Surgery Burn Center of Research Excellence,Texas Tech University Health Sciences Center

Journal (Immunology and Infection)

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Katharina M. Scheurlen¹, Dylan L. Snook¹, Sarah A. Gardner¹, Maurice R. Eichenberger¹, Susan Galandiuk¹

¹Price Institute of Surgical Research, Department of Surgery,University of Louisville

Journal (Immunology and Infection)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Chelsea D. Landon^1,2, Gabriella Dancourt¹, Vivian Shing², Herman F. Staats^2,3,4

¹Division of Laboratory Animal Resources,Duke University Medical Center, ²Department of Pathology,Duke University Medical Center, ³Department of Immunology,Duke University Medical Center, ⁴Duke Human Vaccine Institute,Duke University Medical Center

Journal (Immunology and Infection)

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Anil Kumar¹, Surekha P.¹, Sahil Gulati², Somnath Dutta¹

¹Molecular Biophysics Unit,Indian Institute of Science, ²Gatan Inc.

Journal (Immunology and Infection)

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Amanda R. Paixão*¹, Beatriz R. S. Dias*¹, Luana C. Palma¹, Natália M. Tavares¹, Cláudia I. Brodskyn¹, Juliana P. B de Menezes¹, Patricia S. T. Veras^1,2

¹Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, ²National Institute of Science and Technology of Tropical Diseases - National Council for Scientific Research and Development (CNPq)

Journal (Immunology and Infection)

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Jodi F. Evans¹, Anthony E. Ricigliano¹, Anthony V. Morante¹, Emily Martinez¹, Darlisy Vargas¹, Jonah Thyagaraj¹

¹Molloy College

Journal (Immunology and Infection)

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Camila Gachet-Castro*¹, Lays Adrianne Mendonça Trajano-Silva*¹, Munira Muhammad Abdel Baqui¹

¹Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School,University of Sao Paulo, Ribeirao Preto, SP, Brazil

Journal (Immunology and Infection)

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Katharine M. Ng^1,2, Carolina Tropini^1,2,3

¹School of Biomedical Engineering,University of British Columbia, ²Department of Microbiology and Immunology,University of British Columbia, ³Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Journal (Immunology and Infection)

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Shalhevet Azriel*^1,2, Hadar Bootz*^1,2, Alon Shemesh*^1,2, Sivan Amidror^1,2, Nissan Yissachar^1,2

¹The Goodman Faculty of Life Sciences,Bar-Ilan University, ²Bar-Ilan Institute of Nanotechnology and Advanced Materials,Bar-Ilan University

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