JOVE JOURNAL

Biochemie

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A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Jenny L. Carey¹, Olivia H. Cox¹, Fayaz Seifuddin¹, Leonard Marque¹, Kellie L.K. Tamashiro¹, Peter P. Zandi^1,3, Gary S. Wand^1,2, Richard S. Lee¹

¹Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, ²Department of Medicine,Johns Hopkins School of Medicine, ³Department of Mental Health,Johns Hopkins School of Public Health

Journal (Biochemistry)

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Ryan C. Oliver¹, Swe-Htet Naing², Kevin L. Weiss¹, Sai Venkatesh Pingali¹, Raquel L. Lieberman², Volker S. Urban¹

¹Neutron Scattering Division,Oak Ridge National Laboratory, ²School of Chemistry and Biochemistry,Georgia Institute of Technology

Journal (Biochemistry)

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Sofiia Karchugina¹, Jonathan Chernoff¹

¹Fox Chase Cancer Center

Journal (Biochemistry)

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Imen Bouchnak¹, Lucas Moyet¹, Daniel Salvi¹, Marcel Kuntz¹, Norbert Rolland¹

¹Université Grenoble Alpes, INRA, CNRS, CEA

Journal (Biochemistry)

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng^1,3, Xing Chen^1,2,3,4,5

¹College of Chemistry and Molecular Engineering,Peking University, ²Beijing National Laboratory for Molecular Sciences,Peking University, ³Peking-Tsinghua Center for Life Sciences,Peking University, ⁴Synthetic and Functional Biomolecules Center,Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

Journal (Biochemistry)

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Sumino Yanase^1,2, Kayo Yasuda³, Naoaki Ishii³

¹Department of Health Science, School of Sports & Health Science,Daito Bunka University, ²Department of Molecular Life Science, School of Medicine,Tokai University, ³Department of Health Management, School of Health Study,Tokai University

Journal (Biochemistry)

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Chayan Bhattacharya*¹, Aninda Sundar Dey*¹, Navid J. Ayon*¹, William G. Gutheil¹, Mridul Mukherji¹

¹Division of Pharmaceutical Sciences, School of Pharmacy,University of Missouri-Kansas City

Journal (Biochemistry)

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Antonio E. Chambers*¹, Adam E. Richardson*¹, David F. Read², Thomas J. Waller³, Douglas A. Bernstein¹, Philip J. Smaldino¹

¹Department of Biology,Ball State University, ²Department of Genome Sciences,University of Washington, ³Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Journal (Biochemistry)

Rapid Isolation of the Mitoribosome from HEK Cells

Shintaro Aibara¹, Juni Andréll¹, Vivek Singh¹, Alexey Amunts¹

¹Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Journal (Biochemistry)

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Stacy N. Uchendu¹, Angelika Rafalowski¹, Erin F. Cohn¹, Luke W. Davoren¹, Erika A. Taylor¹

¹Department of Chemistry,Wesleyan University

Journal (Biochemistry)

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Roberto Pech-Kú¹, J. Armando Muñoz-Sánchez¹, Miriam Monforte-González¹, Felipe Vázquez-Flota¹, Beatriz A. Rodas-Junco², S.M. Teresa Hernández-Sotomayor¹

¹Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán, ²CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería,Universidad Autónoma de Yucatán

Journal (Biochemistry)

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology,University of California - Davis

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