JOVE JOURNAL

Biochemie

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Journal (Biochemistry)

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Ibrahim B. Durowoju¹, Kamaljit S. Bhandal¹, Jian Hu¹, Bruce Carpick¹, Marina Kirkitadze¹

¹Analytical Research & Development,Sanofi Pasteur Limited

Journal (Biochemistry)

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Lisa M. Meints¹, Anne W. Poston¹, Brent F. Piligian¹, Claire D. Olson¹, Katherine S. Badger², Peter J. Woodruff², Benjamin M. Swarts¹

¹Department of Chemistry and Biochemistry,Central Michigan University, ²Department of Chemistry,University of Southern Maine

Journal (Biochemistry)

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Thilo Dörfler*¹, Tobias Eilert*¹, Carlheinz Röcker¹, Julia Nagy¹, Jens Michaelis¹

¹Institute of Biophysics,Ulm University

Journal (Biochemistry)

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Irena Roci^1,2, Hector Gallart-Ayala³, Jeramie Watrous⁴, Mohit Jain⁴, Craig E. Wheelock³, Roland Nilsson^1,2

¹Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, ²Center for Molecular Medicine,Karolinska Institutet, ³Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, ⁴Department of Medicine,University of California San Diego

Journal (Biochemistry)

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Zheng Ma*¹, Victor Fung*¹, Iván D'Orso¹

¹Department of Microbiology,The University of Texas Southwestern Medical Center

Journal (Biochemistry)

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Yuno Natsume¹, Hsin-i Wen², Tong Zhu², Kazumi Itoh¹, Li Sheng², Kensuke Kurihara^2,3,4

¹Department of Mathematical and Physical Sciences, Faculty of Science,Japan Women's University, ²Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences, ³Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science,National Institutes of Natural Sciences, ⁴Research Center for Complex Systems Biology,The University of Tokyo

Journal (Biochemistry)

Laboratory Scale Production and Purification of a Therapeutic Antibody

Zehra Elgundi¹, Vicki Sifniotis¹, Mouhamad Reslan¹, Esteban Cruz¹, Veysel Kayser¹

¹Faculty of Pharmacy,University of Sydney

Journal (Biochemistry)

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto³, Hidesato Ogawa¹, Hiroaki Taniguchi^3,4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

Journal (Biochemistry)

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Xinzhou Zhu¹, Andrea Zelmer¹, Sven Wellmann^1,2

¹University Children's Hospital Basel (UKBB),University of Basel, ²Department of Clinical Research,University of Basel

Journal (Biochemistry)

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Carlos Eduardo Espinosa-de la Garza*¹, Rodolfo Daniel Salazar-Flores*¹, Néstor Octavio Pérez¹, Luis Francisco Flores-Ortiz¹, Emilio Medina-Rivero¹

¹Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Journal (Biochemistry)

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Gatikrushna Singh*¹, Sarah M. Fritz*², Arnaz Ranji², Deepali Singh³, Kathleen Boris-Lawrie^1,2

¹Department of Veterinary & Biomedical Sciences,University of Minnesota, ²Department of Veterinary Biosciences,Ohio State University, ³School of Biotechnology,Gautam Buddha University

Journal (Biochemistry)

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Jonathan Fischer¹, Roger A. Johnson², Elizabeth Boon¹

¹Department of Chemistry,Stony Brook University, ²Department of Physiology and Biophysics,Stony Brook University

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