Encyclopedia Of Experiments

Drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Encyclopedia Of Experiments

Drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Sections

Embryo

Larva

Pupa

Adult

Embryo

Larva

Pupa

Adult

Embryo

Microinjection of Drosophila Nurse Cells

A Method of Intracellular Delivery

Drosophila Egg Collection and Dechorionation

A Method to Remove the Outermost Egg Layer

Preparation of Fixed Drosophila Oocytes for Immunostaining

A High-Throughput Method to Fix and Remove the Outer Membrane

Chorion and Vitelline Membrane Mechanical Removal

A Method to Prepare Drosophila Oocytes for Direct Observation

Microinjection of Live Drosophila Embryos

Early Delivery of Reagents to the Developing Embryo

Larva

Drosophila Burrowing and Tunneling Assay

A Method to Assess Tissue Hypoxia in Fly Larvae

Drosophila Larva Imaginal Disc Dissection

A Method to Observe Developing Epithelia

Drosophila Neuromuscular Junction (NMJ) Quantification

A Method to Assess Synaptic Morphology and Function

Two-Photon Laser-Induced Neural Injury

A Method to Observe Axon Degeneration and Regeneration in Drosophila Larvae

Larval Fillet Preparation

A Method to Visualize Intact Sensory Neurons and Associated Epidermal Cells

Cuticle Disruption

A Method to Collect Hemolymph from Drosophila Larvae

Light Spot Assay

A Method to Investigate Drosophila Phototactic Behavior

Larval Ring Gland Dissection

Isolation of Developmental Endocrine Organs from Drosophila

Local Heat Probe Assay

A Method to Assess Nociception of High Temperatures in Drosophila Larvae

Temperature Gradient Assay

A Method to Test Temperature Preference in Drosophila Larvae

Larval Lymph Gland Dissection

Isolating the Drosophila Hemocyte-Producing Organ

Pupa

Insect Puparium Removal

A Method for Pupal Investigation

Dissection of the Eye-Brain Complex from Fly Pupae

A Method to Isolate Retinal Tissue

Drosophila Late Pupa Indirect Flight Muscle (IFM) Dissection

A Method for High-Throughput Tissue Collection

Adult

Drosophila Courtship Conditioning

A Method to Test Learning and Memory In Flies

Fly Climbing Assay

A Method to Test General Locomotion in Drosophila melanogaster

Drosophila Male Accessory Gland Dissection

A Method to Isolate Secondary Cells

Drosophila melanogaster Ovary Dissection

A Technique for Ex Vivo Tissue Preparation

Malpighian Tubule Dissection from an Adult Fly

Isolating an Osmoregulatory and Excretory Organ

Island Assay

A High-Throughput Method to Evaluate Drosophila Locomotor Behavior

Lifespan Protocol for Drosophila melanogaster

Generating Survival Curves to Identify Differences in Fly Longevity

Feeding Test

Assessment of Feeding Preferences in Drosophila melanogaster

Mounting Adult Drosophila Brains

A Method to Prepare Slides for Confocal Imaging

Screening for Genomic Modifications

A Method to Identify CRISPR-Generated Mutants in Drosophila

Chemical Drying

Biological Sample Preparation for Scanning Electron Microscopy (SEM)

Embedding Adult Drosophila Heads in Paraffin

Preparing Tissue for Histology

Injection of Adult Drosophila Flies

A Method of Compound or Label Delivery

Taste Preference Assay

A Method for Measuring Feeding Behavior in Drosophila

The CApillary FEeder (CAFE) Assay

A Method to Track Food Consumption and Preference in Drosophila

Drosophila Adult Brain Dissection

A Method in Fly Neurobiology

Counting Colony Forming Units (CFUs)

Quantifying Bacteria from Fly Bodies

Processing Insect Legs for Fluorescence Microscopy

A Method to Preserve Neuromuscular Structures for Imaging

Tracking Freely Walking Flies

A Method to Assess Locomotion in Drosophila

Fly Aspirator Tube

A Simple Tool to Transfer Live Drosophila

Drosophila In Vivo Calcium Imaging

A Method for Functional Imaging of Neuronal Activity

Four-Way Olfactometer Assay

A Method to Assess Odorant-Cued Responses in Drosophila

Drosophila Aggression Assay

A Method to Test Behavioral Plasticity in Flies

Drosophila Y-maze Assay

A Method to Assess Olfactory Responses in Flies

Drosophila Activity Monitor (DAM)

A Method to Measure Locomotor Activity in Flies

Drosophila Optogenetics

A Method to Manipulate Neuronal Circuits

Dissection of the Midgut from an Adult Fly

A Method for Isolating the Digestive Tract

Preparation of Drosophila Heart

A Technique to Study Heart Physiology in the Adult Fly

Drosophila Egg-Laying Preference Assay

A Method to Test Decision-Making in Flies