Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells

Qianwen Zheng; Xiaolong Xie; Yao Li; Chengbo Ai; Siyu Pu; Jing Chen

doi:10.3791/66458

Improved Procedure to Obtain Granulosa Cell-Free Stage I Zebrafish Oocytes

July 26, 2024

Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells

01:56 min

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Qianwen Zheng, Xiaolong Xie, Yao Li, Chengbo Ai, Siyu Pu, Jing Chen

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine,Sichuan University

Summary

This protocol describes a modified procedure for rapidly isolating clean stage I oocytes in zebrafish devoid of granulosa cells, thereby providing a convenient method for oocyte-specific research.

Improved Procedure to Obtain Granulosa Cell-Free Stage I Zebrafish Oocytes

Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells

Summary

Article