Journal
Immunologie und Infektion
Histological Staining of Spinal Cord for Assessing EAE Disease Severity

Histological Staining of Spinal Cord for Assessing EAE Disease Severity

February 23, 2024

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Harvesting and Fixing Mouse Brain and Spinal Cord Tissues for Histological Scoring of Experimental Autoimmune Encephalomyelitis

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Paraffin Embedding and Histopathological Scoring of Demyelinating Lesions and Perivascular Cuffs in Mouse Brain and Spinal Cord Tissues

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1Department of Immunology,University of Toronto, 2Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, 3Sickkids Research Institute,The Hospital for Sick Children, 4BARLO MS Centre,St. Michael’s Hospital, 5Women’s College Research Institute,Women’s College Hospital

Summary

Experimental autoimmune encephalomyelitis (EAE) serves as an animal model of multiple sclerosis. This article describes an approach for scoring spinal cord inflammation, demyelination, and axonal injury in EAE. Additionally, a method to quantify soluble neurofilament light levels in the mice serum is presented, facilitating the assessment of axonal injury in live mice.

Article