Mapping the Tumor Immune Landscape: An In-Depth Multiplex IHC Analysis Protocol

Mapping the Tumor Immune Landscape: An In-Depth Multiplex IHC Analysis Protocol

August 18, 2023

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Workflow for Immune Cells Detection Using the Machine Learning Algorithm ImmuNet

Mark A. J. Gorris^1,2, Evgenia Martynova*^1,3, Mark W. D. Sweep*^1,4, Iris A. E. van der Hoorn^1,5, Shabaz Sultan^1,3, Mike J. D. E. Claassens¹, Lieke L. van der Woude^1,2,6, Kiek Verrijp^1,2,6, Carl G. Figdor^1,3, Johannes Textor^1,3, I. Jolanda M. de Vries¹

¹Department of Medical BioSciences,Radboudumc, ²Division of Immunotherapy, Oncode Institute,Radboudumc, ³Data Science, Institute for Computing and Information Sciences,Radboud University, ⁴Department of Medical Oncology,Radboudumc, ⁵Department of Pulmonary Diseases,Radboudumc, ⁶Department of Pathology,Radboudumc

Summary

This protocol describes in detail how immune cell characterization of the tumor microenvironment using multiplex immunohistochemistry is carried out.

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