Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Lisa  A. Krattiger; Maria Mitsi; Benjamin R. Simona; Martin Ehrbar

doi:10.3791/65413

Journal

Biotechnik

3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening

May 19, 2023

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

01:15 min

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889 Views

Establishing the 3D Stromal Monoculture

01:46 min

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239 Views

Establishing the 3D Stromal-Endothelial Cell Co-Culture

01:48 min

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311 Views

Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture

02:34 min

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263 Views

Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

03:44 min

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342 Views

Lisa A. Krattiger¹, Maria Mitsi², Benjamin R. Simona², Martin Ehrbar¹

¹Department of Obstetrics,University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

Summary

An in vitro model of the bone marrow vascular niche is established by seeding mesenchymal and endothelial cells onto pre-cast 3D PEG hydrogels. The endothelial networks, ECM components, and ALP activity of the niches vary depending on the growth factor used. The platform can be used for advanced cancer models.