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Preparation of 2-dGuo-Treated Thymus Organ Cultures

JoVE 杂志
生物学

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JoVE 杂志生物学

Preparation of 2-dGuo-Treated Thymus Organ Cultures

Preparation of 2-dGuo-Treated Thymus Organ Cultures

DOI:

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07:50 min

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August 28, 2008

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William Jenkinson, Eric Jenkinson, Graham Anderson

¹MRC Center for Immune Regulation,University of Birmingham

Chapters

00:00Title
00:15Introduction
01:09Preparation of 2-dGuo Treated Alymphoid Thymus Organ Cultures
06:50Conclusion

Summary

自动翻译

This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.

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2-dGuo-Treated Thymus Organ Cultures Thymus T-cell Precursors Stromal Cells Cortical Epithelial Cells Medullary Epithelial Cells T-cell Development In Vitro Culture Systems Thymus Organ Culture Mouse Embryonic Thymus Lobes Haemopoeitic Elements 2-deoxyguanosine Thymic Microenvironments Alymphoid Thymus Lobes Haemopoietic Elements Recipient Mice Reaggregate Thymus Organ Cultures

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