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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

DOI:
10.3791/832-v

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03:50 min

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June 10, 2008

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, ,
1David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:00Title
  • 00:20Splitting hESCs From Matrigel to Matrigel
  • 03:23Conclusion

Summary

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 3 of 3.

Tags

MEFsMatrigelHESCsFeeder Cell-free ConditionsPassageGrowthPluripotencyImmunofluorescence Microscopy

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