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İnsan Kaynaklı Pluripotent Kök Hücrelerden Osteoklastların Farklılaşması ve Karakterizasyonu
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发育生物学
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JoVE 杂志 发育生物学
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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İnsan Kaynaklı Pluripotent Kök Hücrelerden Osteoklastların Farklılaşması ve Karakterizasyonu

1,168 Views

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10:52 min

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March 22, 2024

DOI:

10.3791/66527-v

DOI:
10.3791/66527-v

•

10:52 min
March 22, 2024

•

68 Views
, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Summary

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Bu protokol, insan osteoklastlarının indüklenmiş pluripotent kök hücrelerden (iPSC'ler) ayırt edilmesini sunar ve osteoklastların ve osteoklast öncüllerinin karakterizasyonu için yöntemleri açıklar.

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Cite this Article

Blümke, A., Simon, J., Leber, E., Scatena, M., Giachelli, C. M. Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (205), e66527, doi:10.3791/66527 (2024).

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