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Fabricação de grade de afinidade por estreptavidina para preparação de amostras de microscopia crio-eletrônica

JoVE 杂志
生物化学

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JoVE 杂志生物化学

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Fabricação de grade de afinidade por estreptavidina para preparação de amostras de microscopia crio-eletrônica

3,769 Views

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01:15 min

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December 29, 2023

DOI:

10.3791/66197-v

DOI:

10.3791/66197-v

•

01:15 min

December 29, 2023

•

37 Views

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

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