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胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养
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发育生物学
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JoVE 杂志 发育生物学
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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胎儿小鼠骨骼肌3D脱细胞基质的制备用于细胞培养

1,024 Views

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07:44 min

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March 03, 2023

DOI:

10.3791/65069-v

DOI:
10.3791/65069-v

•

07:44 min
March 03, 2023

•

20 Views
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1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences,University of Lisbon

Summary

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在这项工作中，优化了脱细胞方案以获得胎鼠骨骼肌的脱细胞基质。C2C12成肌细胞可以在这些基质中定植，增殖和分化。这种 体外 模型可用于研究骨骼肌疾病（如肌肉萎缩症）背景下的细胞行为。

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Cite this Article

Gameiro dos Santos, P., Soares, A. R., Thorsteinsdóttir, S., Rodrigues, G. Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture. J. Vis. Exp. (193), e65069, doi:10.3791/65069 (2023).

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