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Evaluering af hjertekontraktilitetsmodulationsterapi i 2D humane stamcelleafledte kardiomyocytter
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
DOI:

08:47 min

December 16, 2022

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Chapters

  • 00:04Introduction
  • 00:50Seeding of Cryopreserved Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs)
  • 01:25Dissociation and Counting of the Pre-Plated hiPSC-CMs
  • 02:49Flexible Hydrogel Substrates Preparation
  • 04:35Contraction Recording and Analysis
  • 06:52Results: Evaluation of CCM in 2D Human Stem Cell-Derived Cardiomyocytes
  • 07:59Conclusion

Summary

自动翻译

Her demonstrerer vi en ikke-invasiv kontraktilitetsevalueringsmetode for hjertemedicinsk udstyr ved hjælp af 2D humant induceret pluripotent stamcelleafledt kardiomyocyt (hiPSC-CM) monolag, belagt på et fleksibelt substrat kombineret med videobaseret mikroskopi. Dette værktøj vil være nyttigt til in vitro-evaluering af kontraktile egenskaber af hjerteelektrofysiologiske enheder.

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