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生物化学
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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
JoVE 杂志
生物化学
Author Produced
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JoVE 杂志 生物化学
Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

1,185 Views

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09:03 min

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March 17, 2023

DOI:

10.3791/64707-v

DOI:
10.3791/64707-v

•

09:03 min
March 17, 2023

•

13 Views
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1Bioprocessing Technology Institute (BTI),Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

Summary

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Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

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Cite this Article

Lam, A. T. L., Jayaraman, P., Becker, A., Lim, R., Teo, K. L., Ng, J., Oh, S. Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow. J. Vis. Exp. (193), e64707, doi:10.3791/64707 (2023).

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