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Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome
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生物学
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JoVE 杂志 生物学
Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome

DOI:
10.3791/64605-v

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08:38 min

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March 31, 2023

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Chapters

  • 00:05Introduction
  • 00:39Collecting Worm Population for Gut Microbiome Analyses
  • 02:08Setting Up the Large Particle Sorter and Autosampler
  • 03:43Sorting of Caenorhabditis elegans by Gut Microbiome Features
  • 06:05Results: Profiling Gut Microbiome Colonization
  • 07:50Conclusion

Summary

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Caenorhabditis elegans is a powerful model to examine the molecular determinants driving host-microbiome interactions. We present a high throughput pipeline profiling the single animal levels of gut microbiome colonization together with key aspects of the C. elegans physiology.

Tags

Keywords: Flow CytometryGut MicrobiomeCaenorhabditis ElegansFluorescenceHigh-throughputReal-time MonitoringPhysiologyLevamisoleBleachParalysisWashAutosamplerLasersChannelsFlow Cytometer

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