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Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes

Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes

DOI:
10.3791/64394-v

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09:17 min

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August 09, 2022

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, , , , , , ,
1AnaBios Corporation

Chapters

  • 00:05Introduction
  • 00:51Laminin Coating Preparation (One Day Before Measuring Contractility)
  • 01:28Preparation of Test Compounds (On the Day of Measuring Contractility)
  • 02:42Plating of Cardiomyocytes (On the Day of Measuring Contractility)
  • 03:30Recording of Cardiomyocyte Contractility
  • 05:13Analysis of Contractility Data
  • 07:44Results: Validating Human Cardiomyocyte Contractility Measurement
  • 08:26Conclusion

Summary

自动翻译

自动翻译

This protocol describes how to measure contractility in adult human primary cardiomyocytes from donor hearts with the MyoBLAZER system, a reliable platform for assessing drug-induced changes in contractility during preclinical development.

Tags

Heart ContractilityAdult Human Primary CardiomyocytesOptical RecordingDrug EffectsHeart FailureDrug-induced Contractility RisksLamininDMSOGravity Flow Perfusion SystemMicroscope

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