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堆肥微观世界作为秀丽隐杆线虫微生物组研究的微生物多样化、类似自然的环境
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans
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堆肥微观世界作为秀丽隐杆线虫微生物组研究的微生物多样化、类似自然的环境

DOI:
10.3791/64393-v

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07:19 min

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September 13, 2022

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, ,
1Department of Integrative Biology,University of California, Berkeley

Chapters

  • 00:04Introduction
  • 00:36Preparation of Compost
  • 01:20Preparation of Compost Microcosms and Raising Worms in Compost Microcosms
  • 02:39Preparing a Baermann Funnel, Harvesting Worms from Microcosms, and Collecting the Respective Soil Samples
  • 04:17Washing and Surface Sterilization of the Harvested Worms
  • 05:43Results: Comparisons of Worm Gut Microbiomes and Environmental Communities
  • 06:26Conclusion

Summary

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堆肥微观世界将自然界中发现的微生物多样性带入实验室，以促进 秀丽隐杆线虫的微生物组研究。这里提供了用于建立微观实验的协议，实验展示了调节环境微生物多样性的能力，以探索环境微生物多样性与蠕虫肠道微生物组组成之间的关系。

Tags

Keywords: Compost MicrocosmsCaenorhabditis ElegansHost-microbiome InteractionsNatural-like EnvironmentMicrobial DiversityWorm IsolationBaermann FunnelM9 MediumL1 Larvae

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