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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

3,234 Views

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10:57 min

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August 24, 2022

DOI:

10.3791/64385-v

DOI:
10.3791/64385-v

•

10:57 min
August 24, 2022

•

6 Views
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1Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science,The University of Tokyo, 2Research Institute for Microbial Diseases,Osaka University

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Ozawa, M., Emori, C., Ikawa, M. CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models. J. Vis. Exp. (186), e64385, doi:10.3791/64385 (2022).

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