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分离小鼠肠系膜流动淋巴液以量化膳食脂质吸收和乳糜微粒分泌的体内动力学

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

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分离小鼠肠系膜流动淋巴液以量化膳食脂质吸收和乳糜微粒分泌的体内动力学

1,779 Views

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06:14 min

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November 30, 2022

DOI:

10.3791/64338-v

DOI:

10.3791/64338-v

•

06:14 min

November 30, 2022

•

31 Views

Nikolaos L. Dedousis¹, Lihong Teng¹, Alison B. Kohan¹

¹Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine,University of Pittsburgh

Summary

本协议描述了一种详细的手术方案，用于分离流动的肠淋巴以响应小鼠十二指肠内营养输注。这允许对响应各种实验营养素的总肠道脂质吸收和乳糜微粒合成和分泌进行生理测定。

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