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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
JoVE Journal
Biology
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JoVE Journal Biology
The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

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1,837 Views

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06:14 min

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November 30, 2022

DOI:

10.3791/64338-v

DOI:
10.3791/64338-v

•

06:14 min
November 30, 2022

•

31 Views
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1Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine,University of Pittsburgh

Summary

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The present protocol describes a detailed surgical protocol for isolating flowing intestinal lymph in response to intraduodenal nutrient infusions in mice. This allows for the physiological determination of total intestinal lipid absorption and chylomicron synthesis and secretion in response to various experimental nutrients.

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Cite this Article

Dedousis, N. L., Teng, L., Kohan, A. B. The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion. J. Vis. Exp. (189), e64338, doi:10.3791/64338 (2022).

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