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1Department of Clinical Immunology,Wroclaw Medical University, 2Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences,Jagiellonian University Medical College, 3Chair of Biomedical Sciences, Faculty of Health Sciences,Jagiellonian University Medical College
Chapters
- 00:04Introduction
- 00:59Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)
- 02:19Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay
- 03:48Vascular Permeability Test
- 04:43Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement
- 05:16Adoptive Transfer of the CHS-Effector Cells
- 06:41Results: Study of the Contact Hypersensitivity Reaction in Mice
- 07:42Conclusion
A hipersensibilidade de contato (CHS) é um modelo experimental de murina de dermatite de contato alérgico (ACD). O CHS baseia-se na sensibilização com hapten reativo pintando a pele raspada do peito e abdômen, com um subsequente desafio da pele do ouvido com um hapten diluído, causando uma reação de inchaço que é avaliada de várias maneiras.
