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接触超敏反应作为过敏性接触性皮炎的小鼠模型
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
DOI:

08:25 min

September 26, 2022

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Chapters

  • 00:04Introduction
  • 00:59Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)
  • 02:19Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay
  • 03:48Vascular Permeability Test
  • 04:43Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement
  • 05:16Adoptive Transfer of the CHS-Effector Cells
  • 06:41Results: Study of the Contact Hypersensitivity Reaction in Mice
  • 07:42Conclusion

Summary

自动翻译

接触性超敏反应(CHS)是过敏性接触性皮炎(ACD)的小鼠实验模型。CHS基于通过涂上胸部和腹部的剃须皮肤对反应性半抗原的致敏,随后用稀释的半抗原进行耳朵皮肤激发,引起肿胀反应,以各种方式评估。

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