Journal
/
免疫与感染
/
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

DOI:
10.3791/64288-v

•

10:16 min

•

August 17, 2022

•

, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Chapters

  • 00:05Introduction
  • 01:19In Vitro Transcription (IVT) of Chemically Modified mRNA
  • 02:55Generation of IVT mRNA/PP-sNps
  • 05:22Preparation of the Cells for Transfection and Transfection of the Cultured Cells
  • 07:04Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
  • 07:53Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
  • 09:12Conclusion

Summary

自动翻译

自动翻译

A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.

Tags

MRNA TransfectionPeptide-poloxamine NanoparticlesMucosal MRNA VaccineLung CancerCystic FibrosisIn Vitro TranscriptionCapped MRNAAgarose Gel ElectrophoresisPoloxamine 704Synthetic Peptide

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Seven Steps to Stellate Cells

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Read Article